Efek Galing (Cayratia trifolia) Terhadap Kadar MDA Darah pada Tikus (Rattus norvegicus) yang Diinduksi Parasetamol
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Stres oksidatif terjadi ketika proses oksidasi melampaui kapasitas antioksidan tubuh akibat terganggunya keseimbangan antara keduanya, ditandai dengan peningkatan kadar malondialdehid (MDA) darah. Tanaman Galing diketahui mengandung flavonoid dengan aktivitas antioksidan. Penelitian ini dirancang untuk mengevaluasi potensi ekstrak tanaman Galing dalam mencegah peningkatan kadar MDA darah serta menentukan dosis yang paling efektif pada tikus wistar (Rattus norvegicus) yang diinduksi parasetamol dosis tinggi. Penelitian menggunakan desain post-test only control group dengan pembagian empat kelompok, termasuk kontrol negatif (pakan standar) dan kontrol positif (parasetamol 1.750 mg/kgBB yang diberikan pada hari ke-9), perlakuan 1 (ekstrak Galing 250 mg/kgBB selama 9 hari + parasetamol 1.750 mg/kgBB hari ke-9), dan perlakuan 2 (ekstrak Galing 500 mg/kgBB selama 9 hari + parasetamol 1.750 mg/kgBB hari ke-9). Pengukuran kadar MDA dalam darah dilakukan dengan metode Thiobarbituric Acid Reactive Substances (TBARS). Analisis data dilakukan dengan uji one-way ANOVA. Kelompok kontrol positif menunjukkan peningkatan kadar MDA sebesar 121,75 nmol/L. Perlakuan 1 (250 mg/kgBB) menunjukkan rerata 92,57 nmol/L, sedangkan perlakuan 2 (500 mg/kgBB) menunjukkan rerata 82,29 nmol/L. Uji ANOVA menghasilkan nilai signifikansi p > 0,05, sehingga perbedaan tidak signifikan secara statistik. Berdasarkan hasil tersebut, tanaman Galing tidak berpengaruh dalam mencegah peningkatan kadar malondialdehid (MDA) darah pada tikus wistar (Rattus norvegicus) yang diinduksi parasetamol dosis tinggi. Pemberian dosis tanaman Galing 250 mg/kgBB dan 500mg/kgBB belum memiliki efek preventif secara bermakna secara statistik dalam mencegah peningkatan malondialdehid (MDA) darah.
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Referensi
Agrawal,S., Murray, B., P. And Khaezeni, 2025. Acetaminophen Toxicity. [Online]. Available at : https://www.ncbi.nlm.nih.gov/books/NBK441917/ [Accessed 7 January 2026].
Akhlagi, M. and Foshati, S., 2017. Bioavailability and Metabolism of Flavonoids: A Review Flavonoids Bioavailability Metabolism. International Journal of Nutrition Sciences, 2(4), pp. 180-184.
Andarina, R., & Djauhari, T., 2017. Antioksidan dalam dermatologi. Jurnal Kedokteran dan Kesehatan (JKK), Vol. 4(1), pp. 39–48.
Aisyah, S., Gumelar, A.S., Maulana, M.S. And Amalia, R.A., 2023. Identification Of Mammals Vertebrate Animal Characteristics of White Rat (Rattus Norvegicus) Based on Their Morphology and Anatomy. Prosiding Semhas Bio, Pp.494–493.
Abdallah, A.,Rawan, B., Zaman, A., Aldhafiri, A., Alalawi, A., Omran, F., Baghdadi, H., Abdellah, W., Alsharif, A., Taghfan, S., M Abdel-Rahman, I., El-Sawy, S., Elmoniem, Mahrefam., El Sayed, S. And Abdel-Latif, H., 2023. Original Article Acute Paracetamol Toxicity-Induced Inflammatory and Oxidative Effects Are Relieved by Aleppo Galls: A Novel Experimental Study. [Online] Int J Physiol Pathophysiol Pharmacol, 15(1), 1-11.
Anggraini, W., Rousdy, W., D. and Wardoyo, E., R., P., 2021. Nilai Malondialdehid Hepar Mencit Yang Diinduksi Parasetamol Pada Pemberian Ekstrak Metanol Kulit Kayu Vitex Pubescens Vahl. Jurnal Protobiont, 10(1), Pp.6–11.
Bhuvaneswari, M., 2021. Antioxidant, Antimicrobial and Cytotoxicity Potential Of N-Hexane Extract of Cayratia Trifolia L. Bioinformation, 17(3), Pp.452–459.
Bisht, R., 2018. Antioxidants: A Brief Review. Journal Of Drug Delivery and Therapeutics, 8(6-S), Pp.373–376.
Chaaban Hind, I.I.P.C.C. Celine, G. Mohamed, 2017. The Photostability of Flavanones, Flavonols and Flavones and Evolution of Their Antioxidant Activity. [Online] Available At: Https://Www.Sciencedirect.Com/Science/Article/Abs/Pii/S1010603016305135 [Accessed 23 November 2025].
Cordiano, R., Di Gioacchino, M., Mangifesta, R., Panzera, C., Gangemi, S. And Minciullo, P.L., 2023. Malondialdehyde As A Potential Oxidative Stress Marker for Allergy-Oriented Diseases: An Update. Molecules, 28(16), Pp 1-22.
Debnath, A., Ali, M.A., Newmai, K., Madhuri, P., Azyu, R.Z. and Malsawmsangi, 2024. Effective Management of Wistar Rats in Laboratory Research: A Brief Review. International Journal of Advanced Biochemistry Research, 8(12s), Pp.654–659.
Eghlima, G., Tafreshi, Y.M., Aghamir, F., Ahadi, H. And Hatami, M., 2025. Regional Environmental Impacts on Growth Traits and Phytochemical Profiles of Glycyrrhiza Glabra L. For Enhanced Medicinal and Industrial Use. Bmc Plant Biology, 25(1), 116.
Feriadi., Muhtadi, A. And Barliana, M.E., 2018. Galing (Cayratia Trifolia L.): Sebuah Kajian Biologi, Fitokimia, Dan Aktivitas Farmakologi. Jurnal Farmasi, Sains, Dan Kesehatan, 4(2), Pp.1–5.
Gerriets V, Anderson J, Patel P, Nappe T. M., 2025. Acetaminophen. In: StatPearls. Treasure Island (FL): StatPearls. [Online] Available At: < https://www.ncbi.nlm.nih.gov/books/NBK482369/> [Accessed 18 January, 2026].
Ghazian, G. And Hazmi, A., 2019. Pengaruh Pengeringan Dan Lama Maserasi Dengan Pelarut Ganda Etanol Dan Heksana Terhadap Senyawa Bioaktif Daging Biji Palem Putri (Veitchia Merillii) Effect Of Drying And Maceration Time Using Dual Solvents Ethanol And Hexane on The Bioactive Compounds of Palem Putri Nuts (Veitchia Merillii). Jurnal Pangan Dan Agroindustri, 7(2), Pp.13–23.
Gulcin, I., 2025. Antioksidants, [Online] Available At: Https://Pmc.Ncbi.Nlm.Nih.Gov/Articles/Pmc12402966/ [Accessed 3 January 2026].
Ilyas, M., Muthmainnah, A., Parawansyah, 2017. Efek Hepatoprotektor Ekstrak Etanol Daun Galing (Cayratia Trifolia L.Domin) Pada Tikus Putih (Rattus Norvegicus). Jurnal Warta Farmasi, 6(1), Pp.2089–712.
Inciardi, R.M., Lupi, L., Zaccone, G., Italia, L., Raffo, M., Tomasoni, D., Cani, D.S., Cerini, M., Farina, D., Gavazzi, E., Maroldi, R., Adamo, M., Ammirati, E., Sinagra, G., Lombardi, C.M. And Metra, M., 2020. Cardiac Involvement in A Patient with Coronavirus Disease 2019 (Covid-19). Jama Cardiology, 5(7), Pp.819–824.
Ismirza, Ulfa, H.E.A.Z.M. Saestry, 2025. Perbandingan Penetapan Kadar Flavonoid Total Ekstrak Etanol Daun Asam Kandis Muda Dan Tua (Garcinia Mangostana L.). Jurnal Universitas Mutiara Indonesia, 12(1).
Khalimatussaniah, A., Y. dan Iswari, R., 2015. Pengaruh Suplementasi Madu Kelengkeng Terhadap Kadar Tsa Dan Mda Tikus Putih Yang Diinduksi Timbal (Pb). Jurnal Mipa, 38(2), Pp.108–114.
Kozakiewicz, M., Kornatowski, M., Krzywińska, O. And Kędziora-Kornatowska, K., 2019. Changes In the Blood Antioxidant Defense of Advanced Age People. Clinical Interventions In Aging, 14, Pp.763–771.
Krissanti, I., Hanifa, R. And Dwiwina, R.G., 2023. Efektivitas dan Pengaruh Kombinasi Anestesi Ketamine-Xylazine Pada Tikus (Rattus Norvegicus). Gunung Djati Conference Series, 18, Pp.245–252.
Kunter, I., Zabib, N. And Kosar, M., 2020. A Review of Oxidants and Antioxidants in Biological Systems. Journal Of Pharmaceutical Sciences. 3(1), Pp. 64-72.
Kurniasari, S., Hepi Yanti, A. And Rima Setyawati, T., 2017. Kadar Malondialdehyde Induk Dan Struktur Morfologis Fetus Mencit (Mus Musculus) Yang Diperdengarkan Murottal Dan Musik Rock Pada Periode Gestasi. Journal Protobiont, 6(3), Pp. 89-97.
Mohammed, S., Salunkhe, N., Vishwakarma, K., S., and Maheswari, L., V., 2017. Experimental Validation of Antidiabetic Potential of Cayratia trifolia (L.) Domin: An Indigenous Medicinal Plant. Indian Journal Clinical Biochem, 32(2), pp. 153-162.
Nurfadhila, L., Rahmawati, M., Komala Fitri, N., Nibullah, S.G. And Windari, W., 2023. Journal Of Pharmaceutical and Sciences. Journal Of Pharmaceutical and Sciences, 6(3), Pp.1221–1237.
Nurfitri, M.M., De Queljoey, E. And Datu, O.,S., 2021. Uji Efek Analgetik Ekstrak Etanol Daun Kumis Kucing (Ortosiphon Aristatus (Blume) Miq.) Terhadap Tikus Putih Jantan. Jurnal Pharmacon, 10 (4), Pp. 1155-1161.
Nurhasanah Devika, U. Rengganis, J.F., 2024. The Effect Of Ethanol Concentration Variations on the Total Phenolic and Flavonoid Levels of Bauhinia Purpurea L. Leaf Extract. Journal Of Biotechnology and Natural Science, 4(2), Pp.81–90.
Öksüz E., Yaşar, S., Erten, R., Arihan, O. and Oto, G., 2020. Comparison of high and low dose paracetamol toxicity on liver and brain. Northern Clin Istanbul, 7(6), pp. 541-550.
Parveen, B., Rajinikanth, V. And Narayanan, M., 2025. Natural Plant Antioxidants for Food Preservation and Emerging Trends In Nutraceutical Applications. Discover Applied Sciences, 7(8), 1-29.
Pingli, R., Vemulapalli, S., Narra, U., Polturi, S.V. and Kilaru, N.B., 2023. Diosmin attenuates paracetamol-induced hepato- and nephrotoxicity via inhibition of CY2E1-mediated metabolism in rats. Journal of Applied Pharmaceutical Science, 13(12), pp. 96-104.
Pizzino, G., Irrera,N., Cucinotta, M., Pallio, G., Mannino, F., Arcoraci, V., Squadrito F., Altavilla, D. And Bitto A., 2017. Oxidative Stress: Harms And Benefits For Human Health. [Online] Available At: Https://Pubmed.Ncbi.Nlm.Nih.Gov/28819546/ [Accessed 21 May 2025].
Pooja, G., Shweta, S. And Patel, P., 2025. Oxidative Stress and Free Radicals in Disease Pathogenesis: A Review. Discover Medicine, 2(1), Pp.104.
Prastyo Wati, D.I.S.Y., 2024. Prinsip Dasar Tikus Sebagai Model Penelitian. In: Prinsip Dasar Tikus Sebagai Model Penelitian. Pp.1–20.
Princeton, B., Gayathri, R., Selvaraj, J. And Priya, V.V., 2021. Evaluation Of Anti-Inflammatory and Antioxidant Properties of Cayratia Trifolia. Journal Of Pharmaceutical Research International, 33(6), Pp.174–180.
Putri, T.F., Wasita, B. And Indarto, D., 2023. Administrations Of Butterfly Pea Flower (Clitoria Ternatea L) Extract Reduce Oxidative Stress And Increase Body Weight Of Male Wistar Rats With Diabetes. Journal Amerta Nutrition, 7(3), Pp.400–405.
Qureshi and Ahmed, A., 2015. Evaluation of antioxidant and hepatoprotective potential of Cinnamomum tamala leaves in rats. [Online] Available At: https://journals.lww.com/sjhs/fulltext/2015/04030/evaluation_of_antioxidant_and_hepatoprotective.4.aspx [Accessed 18 January 2025].
Rejeki, S. and Endah, N., 2017. Pembuatan Ekstrak Etanol Dan Penapisan Fitokimia Ekstrak Etanol Kulit Batang Sintok (Cinnamomun Sintoc Bl.). Jurnal hexagro, 1(2), pp. 29-34.
Rejeki, S. Eds., 2018. Ovariektomi Pada Tikus Dan Mencit. In: Ovariektomi Pada Tikus Dan Mencit. Press Airlangga University, 2018.
Riwanti, P. And Izazih, F., 2020. Pengaruh Perbedaan Konsentrasi Etanol Pada Kadar Flavonoid Total Ekstrak Etanol 50,70 Dan 96% Sargassum Polycystum Dari Madura. J-Pham Journal Of Pharmaceutical Care Anwar Medika, 82(2), Pp.2654–8364.
Sies, H., 2020. Oxidative Stress : Concept And Some Practical Aspect. Pubmed Central, 9(9), Pp.852.
Tamawihu, N., E., R., Bodhi, W., Datu, O., S. And Fatimawali, 2024. Uji Aktivitas Antidiabetes Ekstrak Etanol Daun Labu Kuning (Cucurbita Moschata) Pada Tikus Putih Jantan (Rattus Nirvegicus). Jurnal Pharmacon, 13(2), Pp. 619-631.
Tehupuring, S., E., J., Purwaningsari, D. And Pranitasari, N., 2022. Wild Strawberry Fragaria Vesca L. Extracts Hepatoprotective Activities Against Paracetamol-Induced Hepatotoxicity in Male Wistar Rats. Indian Journal of Forensic Medicine & Toxicology, 16(2), Pp.374–380.
Tripathi, R., 2024. Antioxidants: Types, Functions and Usage. International Journal Of Scientific Research In Science and Technology, 11(6), Pp.442–454.
Vanderah. T, W., 2024. Katzung’s Basic & Clinical Pharmacology A Lange Medical Book. 16rd Edition. Arizona : Mcgraw Hill Education.
Widodo, R., Lokajaya, I., Asmungi, 2023. Pelatihan Pemanfaatan Tanaman Galing (Cayratia Trifolia) Sebagai Obat Alternatif Penurun Diabetes. Jurnal Pengabdian Nasional. 3(2), pp. 6-13.
Xu, X., Liu, A., Hu, S. And Ares, I., 2021. Synthetic Phenolic Antioxidants: Metabolism, Hazards and Mechanism of Action. Available at : https://pubmed.ncbi.nlm.nih.gov/33714793/ [Accessed 7 January 2026].
Yosua, K And Dewajanti, A., M., 2017. Efek Ekstrak Bunga Sisir (Illicium Verum) Terhadap Penurunan Kadarmalondialdehida (Mda) Pada Tikus Diabetes Galursprague Dawley. Jurnal Kedokteran Meditek, 23(64), Pp. 7-14.